The Phospho-PLCG2 (Y1217) Polyclonal Antibody (CABP0805) is a valuable tool for researchers studying the phosphorylation of PLCG2 at tyrosine 1217. This phosphorylation event is known to activate PLCG2, a crucial enzyme involved in intracellular signaling pathways regulating immune responses and cell proliferation.Raised in rabbits, this antibody is highly specific to human samples and has been validated for use in Western blot applications. By specifically binding to the phosphorylated form of PLCG2 at tyrosine 1217, researchers can accurately detect and analyze this key protein modification in various cell types.
Understanding the role of PLCG2 phosphorylation at Y1217 is essential for unraveling the intricate mechanisms of immune signaling and cell growth regulation. This antibody provides a reliable tool for investigating the impact of PLCG2 phosphorylation in diseases such as cancer, autoimmune disorders, and inflammatory conditions. Its utility in studying these pathways makes it a valuable asset for researchers working in immunology and oncology fields.
cytoplasm, cytosol, extracellular exosome, perinuclear region of cytoplasm, plasma membrane, ruffle membrane
Calculated MW:
148kDa
Observed MW:
150kDa
The protein encoded by this gene is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate to 1D-myo-inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor. IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane. Mutations in this gene have been found in autoinflammation, antibody deficiency, and immune dysregulation syndrome and familial cold autoinflammatory syndrome 3.
Purification Method:
Affinity purification
Gene ID:
5336
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Raji cells, using Phospho-PLC gamma 2 (PLCG2)-Y1217 Rabbit pAb (CABP0805) at 1:1000 dilution or PLC gamma 2 (PLCG2) antibody (CAB2182). Raji cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
