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Phospho-PLC gamma 1 (PLCG1)-Y783 Rabbit Polyclonal Antibody (CABP0164)

SKU:
CABP0164
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Immunology
€419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-PLC gamma 1 (PLCG1)-Y783 Rabbit Polyclonal Antibody
SKU:CABP0164
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse
Immunogen:A synthetic phosphorylated peptide around Y783 of human PLC gamma 1 (PLCG1) (NP_877963.1?).
Sequence:GFYV E
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:PLC1; NCKAP3; PLC-II; PLC148; PLCgamma1; Phospho-PLC gamma 1 (PLCG1)-Y783
Positive Sample:A-431
Conjugate:Unconjugated
Cellular Localization:Cell projection, lamellipodium, ruffle.
Calculated MW:149kDa
Observed MW:155kDa

The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene.

Purification Method:Affinity purification
Gene ID:5335
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from A-431 cells, using Phospho-PLC gamma 1 (PLCG1)-Y783 Rabbit pAb (CABP0164) at 1:1000 dilution.A-431 cells were treated by EGF (25 μg/mL) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

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