The Phospho-PLCγ1 (Y783) Polyclonal Antibody (CABP0164) is a valuable tool for researchers studying the phosphorylation of PLCγ1 at tyrosine 783. PLCγ1 is a key signaling molecule involved in various cellular processes, including cell growth, differentiation, and immune responses.This antibody, produced in rabbits, is highly specific for detecting the phosphorylation of PLCγ1 at tyrosine 783 in human samples. It has been validated for use in Western blot applications, allowing for the precise detection and analysis of PLCγ1 phosphorylation in different cell types.
Phosphorylation of PLCγ1 at tyrosine 783 is known to play a critical role in regulating downstream signaling pathways involved in cell growth and proliferation. By targeting this specific phosphorylation site, researchers can gain insights into the molecular mechanisms underlying various diseases, including cancer and autoimmune disorders.Overall, the Phospho-PLCγ1 (Y783) Polyclonal Antibody (CABP0164) is a valuable tool for studying the role of PLCγ1 phosphorylation in cell signaling pathways and its implications in disease development and progression.
The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
5335
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from A-431 cells, using Phospho-PLC gamma 1 (PLCG1)-Y783 Rabbit pAb (CABP0164) at 1:1000 dilution.A-431 cells were treated by EGF (25 μg/mL) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
