The Phospho-PLCB3 (S1105) Polyclonal Antibody (CABP0837) is designed for research involving PLCB3, a key enzyme in the phosphoinositide signaling pathway that plays a crucial role in various cellular processes. The antibody, raised in rabbits, is highly specific for phosphorylated PLCB3 at serine 1105 and is validated for use in Western blot and immunohistochemistry applications. It binds specifically to the phosphorylated form of PLCB3, allowing for precise detection and analysis in a variety of cell types.PLCB3 is involved in signal transduction pathways that regulate cell growth, differentiation, and survival, making it a potential target for research in cancer biology and other diseases. Phosphorylation of PLCB3 at serine 1105 has been linked to the activation of downstream signaling cascades involved in cell proliferation and migration.
Understanding the role of phosphorylated PLCB3 in cellular signaling pathways is essential for developing targeted therapies for diseases associated with dysregulated cell signaling.Overall, the Phospho-PLCB3 (S1105) Polyclonal Antibody (CABP0837) is a valuable tool for researchers studying the molecular mechanisms underlying cellular signaling pathways and their implication in disease development. Its high specificity and sensitivity make it a reliable reagent for investigating the role of phosphorylated PLCB3 in various biological processes.
A synthetic phosphorylated peptide around S1105 of human PLC beta 3 (PLCB3) (NP_000923.1).
Sequence:
HNSI S
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
SMDCD; Phospho-PLC beta 3 (PLCB3)-S1105
Positive Sample:
HeLa
Conjugate:
Unconjugated
Cellular Localization:
Membrane, Peripheral membrane protein.
Calculated MW:
139kDa
Observed MW:
150kDa
This gene encodes a member of the phosphoinositide phospholipase C beta enzyme family that catalyze the production of the secondary messengers diacylglycerol and inositol 1,4,5-triphosphate from phosphatidylinositol in G-protein-linked receptor-mediated signal transduction. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
5331
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-PLC beta 3 (PLCB3)-S1105 Rabbit pAb (CABP0837) at 1:1000 dilution. HeLa cells were treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
