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Phospho-PLC gamma 1 (PLCG1)-Y783 Monoclonal Antibody (CABP1340)

SKU:
CABP1340
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Applications:
WB
$167 - $731
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-PLC gamma 1 (PLCG1)-Y783 Monoclonal Antibody
SKU:CABP1340
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Mouse
Immunogen:A synthetic phosphorylated peptide around Y783 of human Phospho-PLC gamma 1 (PLCG1)-Y783 (NP_877963.1).
Sequence:GFYV E
Tested Applications:WB ELISA
Recommended Dilution:WB,1:1000 - 1:5000
Synonyms:PLC1; NCKAP3; PLC-II; PLC148; PLCgamma1; Phospho-PLC gamma 1 (PLCG1)-Y783
Positive Sample:NIH/3T3
Conjugate:Unconjugated
Cellular Localization:cell projection, cytoplasm, cytosol, plasma membrane, ruffle membrane
Calculated MW:149kDa
Observed MW:155kDa

The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene.

Purification Method:Affinity purification
Gene ID:5335
Clone Number:ARC54936
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates, using Phospho-PLC gamma 1 (PLCG1)-Y783 antibody (CABP1340) at1:2000 dilution.NIH/3T3 cells were treated by PDGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.

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