The Phospho-PLC Gamma 1 (PLCG1) Y783 Monoclonal Antibody is a valuable tool for researchers studying intracellular signaling pathways related to phosphorylation events. This antibody, raised in mice, specifically targets the phosphorylated tyrosine 783 residue on the PLCG1 protein, a key regulator of signal transduction in cells.PLCG1 is involved in various cellular processes, including cell growth, differentiation, and immune responses. Phosphorylation of PLCG1 at Y783 is known to activate its enzymatic activity, leading to downstream signaling events. By specifically detecting this phosphorylated form of PLCG1, researchers can gain insights into how this protein contributes to signaling cascades in different cellular contexts.
The Phospho-PLC Gamma 1 Y783 Monoclonal Antibody is optimized for use in techniques such as Western blotting, immunofluorescence, and immunoprecipitation, allowing for the detection and analysis of phosphorylated PLCG1 in a variety of experimental settings. Its high specificity and sensitivity make it an invaluable tool for studying the role of PLCG1 phosphorylation in health and disease, particularly in cancer, inflammation, and immune disorders.
The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
5335
Clone Number:
ARC54936
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using Phospho-PLC gamma 1 (PLCG1)-Y783 antibody (CABP1340) at1:2000 dilution.NIH/3T3 cells were treated by PDGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
