The Phospho-PKD (p-PKCµ S744/748) Polyclonal Antibody (CABP0534) is a valuable tool for researchers studying protein kinase D (PKD), a key player in signal transduction pathways involved in cell growth, differentiation, and apoptosis. This antibody, raised in rabbits, specifically targets the phosphorylated form of PKD at serine residues 744 and 748, allowing for precise detection and analysis of PKD activation in various biological samples.PKD, also known as PKCµ, is a member of the PKC family of kinases and has been implicated in multiple cellular processes, including protein trafficking, gene expression, and cytoskeletal dynamics.
Phosphorylation at serine residues 744 and 748 is a critical event that regulates PKD activity and signaling, making this antibody a valuable tool for researchers studying these pathways.With its high reactivity with human samples and validation for use in Western blot applications, the Phospho-PKD Polyclonal Antibody is ideal for studies in cell signaling, cancer biology, and drug development. By enabling specific detection of phosphorylated PKD, this antibody can help researchers better understand the role of PKD in various biological processes and diseases, ultimately leading to the development of targeted therapies.
The protein encoded by this gene is a serine/threonine protein kinase involved in many cellular processes, including Golgi body membrane integrity and transport, cell migration and differentiation, MAPK8/JNK1 and Ras pathway signaling, MAPK1/3 (ERK1/2) pathway signaling, cell survival, and regulation of cell shape and adhesion.
Purification Method:
Affinity purification
Gene ID:
5587
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of NIH/3T3 cells, using Phospho-PKC mu-S744/748 pAb (CABP0534) at 1:1000 dilution or PRKD1 antibody (CAB16185).NIH/3T3 cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
