The Phospho-PKCdelta (Y311) Polyclonal Antibody (CABP0496) is a key tool for researchers investigating the phosphorylation of PKCdelta at tyrosine 311, a crucial post-translational modification involved in various cellular processes. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards human samples, making it a reliable choice for Western blot applications.PKCdelta is a member of the protein kinase C (PKC) family, known for its involvement in signaling pathways regulating cell proliferation, differentiation, and apoptosis. Phosphorylation of PKCdelta at tyrosine 311 has been linked to its activation and downstream signaling cascades, making it a valuable target for studies in cancer biology, cell signaling, and drug development.
The Phospho-PKCdelta (Y311) Polyclonal Antibody enables precise detection and quantification of phosphorylated PKCdelta in various cell types, providing researchers with insights into the mechanisms underlying its activation and function. With its versatility and reliability, this antibody is an essential tool for exploring the role of PKCdelta phosphorylation in health and disease.
Product Name:
Phospho-PKC delta-Y311 Rabbit Polyclonal Antibody
SKU:
CABP0496
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic phosphorylated peptide around Y311 of rat PKC delta (NP_579841.1).
The protein encoded by this gene is a member of the protein kinase C family of serine- and threonine-specific protein kinases. The encoded protein is activated by diacylglycerol and is both a tumor suppressor and a positive regulator of cell cycle progression. Also, this protein can positively or negatively regulate apoptosis. Defects in this gene are a cause of autoimmune lymphoproliferative syndrome.
Purification Method:
Affinity purification
Gene ID:
5580
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of U937 and Raw264.7 cells, using Phospho-PKC delta-Y311 antibody (CABP0496) at 1:1000 dilution. U-937 cells were treated by PMA/TPA (200nM) for 30 minutes.RAW 264.7 cells were treated by PMA (200 nM) for 30 minutes .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
