The Phospho-PKCDelta (Theta S643/676) Polyclonal Antibody (CABP0567) is a valuable tool for researchers studying protein kinase C delta (PKCδ) signaling pathways. This antibody, raised in rabbits, is highly specific for detecting phosphorylated PKCδ at the theta S643/676 site in human samples and is validated for use in Western blot applications. By binding to the phosphorylated PKCδ protein, this antibody allows for the detection and analysis of PKCδ activation in various cell types.PKCδ is a crucial player in diverse cellular processes, including cell growth, differentiation, and apoptosis. Phosphorylation of PKCδ at the theta S643/676 site is known to regulate its activity and function in signaling cascades.
The ability to specifically detect this phosphorylated form of PKCδ is essential for understanding its role in diseases such as cancer, neurological disorders, and cardiovascular diseases.Researchers studying PKCδ signaling pathways will find the Phospho-PKCDelta (Theta S643/676) Polyclonal Antibody (CABP0567) to be a reliable tool for their experiments. Its high specificity and sensitivity make it a valuable asset for investigating the molecular mechanisms underlying PKCδ activation and its implications in various physiological and pathological conditions.
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. Studies both in human and mice demonstrate that this kinase is involved in B cell signaling and in the regulation of growth, apoptosis, and differentiation of a variety of cell types. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]
Purification Method:
Affinity purification
Gene ID:
5580/5588
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of RAW264.7 cells, using Phospho-PKCδ/θ-S643/676 antibody (CABP0567) at 1:1000 dilution. RAW 264.7 cells were treated by PMA (200 nM) for 30 minutes .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
