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Phospho-PKC delta-S645 Rabbit Polyclonal Antibody (CABP0972)

SKU:
CABP0972
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death
$503
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-PKC delta-S645 Rabbit Polyclonal Antibody
SKU:CABP0972
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic phosphorylated peptide around S645 of human PKC delta (NP_006245.2).
Sequence:RLSY S
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:MAY1; PKCD; ALPS3; CVID9; nPKC-delta; Phospho-PKC delta-S645
Positive Sample:293T
Conjugate:Unconjugated
Cellular Localization:Cell membrane, Cytoplasm, Endoplasmic reticulum, Mitochondrion, Nucleus, Peripheral membrane protein, perinuclear region.
Calculated MW:78kDa
Observed MW:80kDa

The protein encoded by this gene is a member of the protein kinase C family of serine- and threonine-specific protein kinases. The encoded protein is activated by diacylglycerol and is both a tumor suppressor and a positive regulator of cell cycle progression. Also, this protein can positively or negatively regulate apoptosis. Defects in this gene are a cause of autoimmune lymphoproliferative syndrome.

Purification Method:Affinity purification
Gene ID:5580
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from 293T cells, using Phospho-PKC delta-S645 pAb (CABP0972) at 1:1000 dilution or PKC delta antibody (CAB0471).293T cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

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