The Phospho-PKCDelta (S645) Polyclonal Antibody (CABP0972) is a valuable tool for researchers studying the protein kinase C delta isoform and its phosphorylation at serine 645. This antibody, raised in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the phosphorylated form of PKC delta, this antibody allows for the detection and analysis of PKC delta activation in various cell types.PKC delta is a key player in signal transduction pathways involved in cell proliferation, differentiation, and apoptosis. Its phosphorylation at serine 645 has been implicated in regulating its activity and function in various cellular processes.
Research involving PKC delta phosphorylation is essential for understanding its role in diseases like cancer, cardiovascular disorders, and neurological conditions.By using the Phospho-PKCDelta (S645) Polyclonal Antibody, researchers can further investigate the signaling mechanisms involving PKC delta phosphorylation and its potential as a therapeutic target in disease intervention. This antibody is a valuable tool for studies in cell biology, biochemistry, and pharmacology related to PKC delta signaling pathways.
Product Name:
Phospho-PKC delta-S645 Rabbit Polyclonal Antibody
SKU:
CABP0972
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S645 of human PKC delta (NP_006245.2).
The protein encoded by this gene is a member of the protein kinase C family of serine- and threonine-specific protein kinases. The encoded protein is activated by diacylglycerol and is both a tumor suppressor and a positive regulator of cell cycle progression. Also, this protein can positively or negatively regulate apoptosis. Defects in this gene are a cause of autoimmune lymphoproliferative syndrome.
Purification Method:
Affinity purification
Gene ID:
5580
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from 293T cells, using Phospho-PKC delta-S645 pAb (CABP0972) at 1:1000 dilution or PKC delta antibody (CAB0471).293T cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
