The Phospho-PKCalpha (S319) Polyclonal Antibody (CABP0560) is a valuable tool for researchers studying the phosphorylation of PKCalpha at Serine 319, a critical post-translational modification that regulates the activity of this protein kinase. This antibody, produced in rabbits, exhibits high specificity for human samples and has been validated for use in Western blot applications.PKCalpha is a member of the protein kinase C (PKC) family, which plays a key role in various cellular processes, including cell growth, differentiation, and apoptosis. Phosphorylation of PKCalpha at Serine 319 has been implicated in the regulation of its enzymatic activity and signaling pathways.
By targeting this specific phosphorylation site, researchers can gain insights into the molecular mechanisms underlying PKCalpha function in normal and diseased cells.The Phospho-PKCalpha (S319) Polyclonal Antibody is a versatile tool for investigating the role of PKCalpha phosphorylation in different cell types and disease contexts, particularly in cancer research and signal transduction pathways. By enabling the detection and analysis of phosphorylated PKCalpha, this antibody can advance our understanding of the biological functions and therapeutic potential of PKCalpha in various pathophysiological conditions.
Product Name:
Phospho-PKC alpha-S319 Rabbit Polyclonal Antibody
SKU:
CABP0560
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S319 of human PKC alpha (NP_002728.2).
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.
Purification Method:
Affinity purification
Gene ID:
5578
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of HeLa cells, using Phospho-PKC alpha-S319 antibody (CABP0560) at 1:1000 dilution.HeLa cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.Hela cells were treated by ATP(5 mM) at 30℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
