The Phospho-PKC Pan Gamma (T514) Polyclonal Antibody (CABP0613) is a reliable tool for studying the phosphorylation status of protein kinase C (PKC) gamma at threonine 514. PKC gamma is a member of the protein kinase C family, which plays a key role in regulating various cellular processes including cell growth, differentiation, and apoptosis. The phosphorylation of PKC gamma at T514 is known to impact its activity and function.This antibody, raised in rabbits, is specifically targeted towards the phospho-PKC pan gamma (T514) protein and has been validated for use in Western blot applications. It enables researchers to detect and analyze the phosphorylation status of PKC gamma in different cell types, providing valuable insights into its role in signaling pathways and cellular functions.
The phosphorylation of PKC gamma at T514 has been implicated in various diseases and conditions, making it a potential therapeutic target for drug development and personalized medicine. By studying the phosphorylation status of PKC gamma, researchers can gain a better understanding of its role in disease pathogenesis and potentially identify new strategies for treatment.Overall, the Phospho-PKC Pan Gamma (T514) Polyclonal Antibody (CABP0613) is a valuable tool for researchers working in the fields of cell biology, molecular biology, and drug discovery, providing insights into the regulation of PKC gamma and its potential implications in disease processes.
cytoplasm, cytosol, endoplasmic reticulum, extracellular exosome, mitochondrial membrane, mitochondrion, nucleoplasm, perinuclear region of cytoplasm, plasma membrane
Calculated MW:
59-67kDa/74-83kDa
Observed MW:
80kDa
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. [provided by RefSeq, Jul 2008]
Purification Method:
Affinity purification
Gene ID:
5578/5579/5580/5581/5582/5583/5588
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Phospho-PKC (pan) (gamma)-T514 antibody (CABP0613) at 1:1000 dilution. RAW 264.7 cells were treated by PMA (200 nM) for 30 minutes .C6 cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
