Phospho-PKC mu-S910 Polyclonal Antibody (CABP1380)
- SKU:
- CABP1380
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Description
| Product Name: | Phospho-PKC mu-S910 Polyclonal Antibody |
| SKU: | CABP1380 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Human |
| Immunogen: | A synthetic phosphorylated peptide around S910 of human PKC mu (NP_002733.2). |
| Tested Applications: | WB ELISA |
| Recommended Dilution: | WB,1:1000 - 1:5000 |
| Synonyms: | PKD; PKCM; CHDED; PRKCM; PKC-MU; Phospho-PKC mu-S910 |
| Positive Sample: | 293F+PMA/TPA |
| Conjugate: | Unconjugated |
| Cellular Localization: | cell cortex, cytosol, Golgi apparatus, nucleus, plasma membrane, trans-Golgi network |
| Calculated MW: | 102kDa |
| Observed MW: | 115kDa |
The protein encoded by this gene is a serine/threonine protein kinase involved in many cellular processes, including Golgi body membrane integrity and transport, cell migration and differentiation, MAPK8/JNK1 and Ras pathway signaling, MAPK1/3 (ERK1/2) pathway signaling, cell survival, and regulation of cell shape and adhesion.
| Purification Method: | Affinity purification |
| Gene ID: | 5587 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3. |
 | Western blot analysis of lysates from 293F cells using Phospho-PKC mu-S910 Rabbit pAb (CABP1380) at 1:4000 dilution. 293F cells were treated by PMA/TPA (200 nM) at 37℃ for 15 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s. |
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