The Phospho-PKC Alpha (T638) Rabbit Monoclonal Antibody (CABP1045) is a cutting-edge tool for researchers studying protein kinase C (PKC) signaling pathways. This antibody specifically targets phosphorylated PKC alpha at threonine 638, a key phosphorylation site involved in regulating PKC activity. PKC alpha is a member of the PKC family of serine/threonine kinases, which play important roles in cell proliferation, differentiation, and survival. Phosphorylation at T638 has been implicated in various cellular processes, including cell cycle regulation and cell migration. This antibody, validated for use in immunohistochemistry and Western blot applications, provides researchers with a precise tool for studying the role of PKC alpha T638 phosphorylation in different cellular contexts.
With its high reactivity with human samples and specificity for phosphorylated PKC alpha T638, this antibody is ideal for studies in cell signaling, cancer research, and drug development. By targeting this specific phosphorylation site, researchers can gain valuable insights into the function of PKC alpha and its potential as a therapeutic target in various diseases.
Product Name:
Phospho-PKC alpha-T638 Rabbit Monoclonal Antibody
SKU:
CABP1045
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse
Immunogen:
A synthetic phosphorylated peptide around T638 of human PKC alpha (P17252).
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.
Purification Method:
Affinity purification
Gene ID:
5578
Clone Number:
ARC1591
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of C2C12, using Phospho-PKC alpha-T638 Rabbit mAb (CABP1045) at 1:1000 dilution.C2C12 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
