The Phospho PKC Alpha (T638) & PKC Beta II (T1281) Monoclonal Antibody is a key tool for researchers studying protein kinase C (PKC) signaling pathways. PKC enzymes play a vital role in regulating various cellular processes, including cell growth, differentiation, and survival. This antibody, developed through monoclonal antibody technology, specifically recognizes the phosphorylated forms of PKC Alpha at threonine 638 and PKC Beta II at threonine 1281.Validated for use in Western blot applications, this antibody allows for the detection and analysis of phosphorylated PKC Alpha and PKC Beta II proteins in various cell types and tissues.
By targeting these specific phosphorylation sites, researchers can gain insights into the activation and function of PKC enzymes in different biological contexts.The Phospho PKC Alpha (T638) & PKC Beta II (T1281) Monoclonal Antibody is a valuable tool for studying the role of PKC signaling in physiological and pathological conditions, such as cancer, cardiovascular diseases, and neurological disorders. By understanding the phosphorylation status of PKC Alpha and PKC Beta II, researchers can uncover novel therapeutic targets and develop targeted interventions for diseases associated with dysregulated PKC signaling pathways.
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
Purification Method:
Affinity purification
Gene ID:
5578/5579
Clone Number:
ARC58024
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts from 293T cells, using Phospho-PKC alpha-T638 + PKC beta II-T641 Rabbit mAb (CABP1375) at 1:6000 dilution.293T cells were treated by CIP(20μL/400μL) at 37℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 60s.
