The Phospho-PKC Alpha (T410) / PKC Delta (T410) Monoclonal Antibody is a reliable tool for researchers studying protein kinase C (PKC) signaling pathways. This monoclonal antibody, generated using hybridoma technology, specifically targets phosphorylated threonine 410 on PKC alpha and delta isoforms. Validated for use in Western blot applications, this antibody enables the detection and analysis of phosphorylated PKC isoforms in various cell types.PKC alpha and delta are crucial regulators of cell proliferation, differentiation, and apoptosis, making them important targets for cancer research and drug development. Phosphorylation of threonine 410 on these isoforms is known to modulate their activity, highlighting the significance of this antibody in studying the functional implications of PKC phosphorylation in cellular processes.
By offering a high level of specificity and sensitivity, the Phospho-PKC Alpha (T410) / PKC Delta (T410) Monoclonal Antibody provides researchers with a valuable tool for investigating the role of PKC signaling in various biological processes and disease states. Its utility in understanding the regulation of PKC isoforms underscores its importance in advancing research in areas such as cancer biology, signal transduction, and therapeutic target identification.
293T transfected with PRKCA (Human) TPA, 293T transfected with PRKCD (Human) TPA,
Conjugate:
Unconjugated
Cellular Localization:
Cell junction, Cytoplasm, Endosome.
Calculated MW:
76kDa/77kDa
Observed MW:
76-80kDa
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.
Purification Method:
Affinity purification
Gene ID:
5578/5580
Clone Number:
ARC63068
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT)、 293F cells treated by PMA、293F cells treated by PMA after transfected with PRKCA and 293F cells treated by PMA after transfected with PRKCD using Phospho-PKC alpha-T410+PKC delta-T410 Rabbit mAb (CABP1506) at 1:2000 dilution. 293F、293F-PRKCA-Transfected and 293F-PRKCD-Transfected cells were treated by PMA (200 nM) at 37℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
