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Phospho-PKC alpha-T410+PKC delta-T410 Monoclonal Antibody (CABP1506)

SKU:
CABP1506
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Applications:
WB
$167 - $731
Frequently bought together:

Description

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Product Name:Phospho-PKC alpha-T410+PKC delta-T410 Monoclonal Antibody
SKU:CABP1506
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:A synthetic phosphorylated peptide around T410 of human PRKCZ(NP_002735.3).
Sequence:TTST FC
Tested Applications:WB ELISA
Recommended Dilution:WB,1:1000 - 1:3000
Synonyms:AAG6; PKCA; PRKACA; PKCI+/-; PKCalpha; PKC-alpha; Phospho-PKC alpha-T410+PKC delta-T410
Positive Sample:293T transfected with PRKCA (Human) TPA, 293T transfected with PRKCD (Human) TPA,
Conjugate:Unconjugated
Cellular Localization:Cell junction, Cytoplasm, Endosome.
Calculated MW:76kDa/77kDa
Observed MW:76-80kDa

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.

Purification Method:Affinity purification
Gene ID:5578/5580
Clone Number:ARC63068
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from wild type (WT)、 293F cells treated by PMA、293F cells treated by PMA after transfected with PRKCA and 293F cells treated by PMA after transfected with PRKCD using Phospho-PKC alpha-T410+PKC delta-T410 Rabbit mAb (CABP1506) at 1:2000 dilution. 293F、293F-PRKCA-Transfected and 293F-PRKCD-Transfected cells were treated by PMA (200 nM) at 37℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

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