The Phospho-PKA R2 (S99) Rabbit Monoclonal Antibody (CABP1034) is a powerful tool for researchers studying protein kinase A (PKA) signaling pathways. This antibody specifically targets the phosphorylated form of the regulatory subunit RII of PKA at serine 99, allowing for precise detection and analysis in various cell types.PKA is a key player in numerous cellular processes, including cell growth, metabolism, and gene expression. Phosphorylation of the RII subunit at serine 99 is known to regulate PKA activity and influence downstream signaling cascades. By using CABP1034, researchers can effectively study the role of PKA in cellular signaling and its implications in diseases such as cancer, cardiovascular disorders, and neurological conditions.
The high reactivity of this antibody with human samples, along with its validation for Western blot applications, makes it an ideal choice for immunology and cancer research studies. By targeting the phosphorylated form of PKA RII, researchers can gain valuable insights into the intricate regulatory mechanisms governing cellular functions and disease progression. Unlock the mysteries of PKA signaling with CABP1034 and advance your research efforts in molecular biology and drug development.
A synthetic phosphorylated peptide around S99 of human PRKAR2A/PKR2 (P13861).
Sequence:
RVSV C
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Synonyms:
PKR2; PRKAR2; Phospho-PKA RIIα (PRKAR2A)-S99
Positive Sample:
293T+CIP,C6+CIP
Conjugate:
Unconjugated
Cellular Localization:
Cell membrane, Cytoplasm.
Calculated MW:
46kDa
Observed MW:
51kDa
cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of cAMP-dependent protein kinase. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER).
Purification Method:
Affinity purification
Gene ID:
5576
Clone Number:
ARC1579
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of 293T cells, using Phospho-PKA RIIα (PRKAR2A)-S99 Rabbit mAb (CABP1034) at 1:1000 dilution.293T cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
