The PIKFYVE Polyclonal Antibody (CAB6689) is specifically designed for research involving PIKFYVE, a crucial enzyme involved in phosphoinositide metabolism and cellular signaling pathways. This antibody, derived from rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding to the PIKFYVE protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an ideal tool for studies in cell biology and signaling pathways.PIKFYVE is known for its role in regulating cellular processes such as endosomal sorting and membrane trafficking, making it a key player in intracellular signaling cascades.
Dysfunction of PIKFYVE has been implicated in various diseases, including neurodegenerative disorders and metabolic syndromes. Therefore, research into the function and regulation of PIKFYVE is essential for developing targeted therapies for these conditions.Overall, the PIKFYVE Polyclonal Antibody is a valuable tool for researchers investigating the role of PIKFYVE in cellular processes and its implications in disease pathology. Its high specificity and sensitivity make it a reliable choice for studies in molecular biology, cell biology, and drug development.
Product Name:
PIKFYVE Rabbit Polyclonal Antibody
SKU:
CAB6689
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 269-545 of human PIKFYVE (NP_055855.2).
Phosphorylated derivatives of phosphatidylinositol (PtdIns) regulate cytoskeletal functions, membrane trafficking, and receptor signaling by recruiting protein complexes to cell- and endosomal-membranes. Humans have multiple PtdIns proteins that differ by the degree and position of phosphorylation of the inositol ring. This gene encodes an enzyme (PIKfyve; also known as phosphatidylinositol-3-phosphate 5-kinase type III or PIPKIII) that phosphorylates the D-5 position in PtdIns and phosphatidylinositol-3-phosphate (PtdIns3P) to make PtdIns5P and PtdIns(3,5)biphosphate. The D-5 position also can be phosphorylated by type I PtdIns4P-5-kinases (PIP5Ks) that are encoded by distinct genes and preferentially phosphorylate D-4 phosphorylated PtdIns. In contrast, PIKfyve preferentially phosphorylates D-3 phosphorylated PtdIns. In addition to being a lipid kinase, PIKfyve also has protein kinase activity. PIKfyve regulates endomembrane homeostasis and plays a role in the biogenesis of endosome carrier vesicles from early endosomes. The protein plays a key role in cell entry of ebola virus and SARS-CoV-2 by endocytosis Mutations in this gene cause corneal fleck dystrophy (CFD); an autosomal dominant disorder characterized by numerous small white flecks present in all layers of the corneal stroma. Histologically, these flecks appear to be keratocytes distended with lipid and mucopolysaccharide filled intracytoplasmic vacuoles.
Purification Method:
Affinity purification
Gene ID:
200576
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse lung, using PIKFYVE Rabbit pAb (CAB6689) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
