Anti-Phospho-PFKFB2-S483 pAb Antibody (CABP0784)
- SKU:
- CABP0784
- Product Type:
- Antibody
- Applications:
- WB
- IP
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
Anti-Phospho-PFKFB2-S483 pAb Antibody (CABP0784)
The Phospho-PFKFB2 (S483) Polyclonal Antibody (CABP0784) is a valuable tool for researchers studying the phosphorylation of PFKFB2, an enzyme involved in glycolysis regulation. This antibody, produced in rabbits, is highly specific to the phosphorylated form of PFKFB2 at serine 483 and is validated for use in Western blot applications. By detecting and quantifying the phosphorylation status of PFKFB2, researchers can gain insights into its role in metabolic pathways and potential implications in diseases such as cancer, diabetes, and metabolic disorders.PFKFB2 is a key regulator of glycolysis, the process by which cells convert glucose into energy. Phosphorylation of PFKFB2 at serine 483 can modulate its enzymatic activity, impacting glycolytic flux and cellular metabolism.
Understanding the regulation of PFKFB2 phosphorylation is essential for unraveling its contribution to metabolic processes and disease pathology.This polyclonal antibody offers researchers a reliable tool for investigating the phosphorylation status of PFKFB2 in various cell types and experimental conditions, providing valuable insights into its functional roles and potential as a therapeutic target. Studies utilizing this antibody may contribute to advancements in metabolic research and the development of novel treatments for diseases linked to dysregulated glycolysis.
| Antibody Name: | Anti-Phospho-PFKFB2-S483 Antibody |
| Antibody SKU: | CABP0784 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | A synthetic phosphorylated peptide around S483 of human PFKFB2 (NP_006203.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | HeLa |
| Immunogen: | A synthetic phosphorylated peptide around S483 of human PFKFB2 (NP_006203.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | NYSV G |
| Gene ID: | 5208 |
| Uniprot: | O60825 |
| Cellular Location: | |
| Calculated MW: | 54kDa/58kDa |
| Observed MW: | 55KDa |
 | Western blot analysis of extracts of HeLa cells, using Phospho-PFKFB2-S483 antibody (CABP0784) at 1:2000 dilution.HeLa cells were treated by IGF-1 (50 ng/ml) at 37'C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
| Synonyms: | PFKFB2, PFK-2/FBPase-2 |
| Background: | The protein encoded by this gene is involved in both the synthesis and degradation of fructose-2, 6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2, 6-bisphosphate, and a fructose-2, 6-biphosphatase activity that catalyzes the degradation of fructose-2, 6-bisphosphate. This protein regulates fructose-2, 6-bisphosphate levels in the heart, while a related enzyme encoded by a different gene regulates fructose-2, 6-bisphosphate levels in the liver and muscle. This enzyme functions as a homodimer. Two transcript variants encoding two different isoforms have been found for this gene. |
| UniProt Protein Function: | PFKFB2: a bifunctional heterodimeric enzyme involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. Regulates fructose-2,6-bisphosphate levels in the heart, while a related enzyme encoded by a different gene regulates fructose-2,6-bisphosphate levels in the liver and muscle. Two splice-variant isoforms have been described. |
| UniProt Protein Details: | Protein type:EC 3.1.3.46; Carbohydrate Metabolism - fructose and mannose; EC 2.7.1.105; Kinase, other; Phosphatase (non-protein); Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 1q31 Cellular Component: cytosol Molecular Function:6-phosphofructo-2-kinase activity; protein binding; protein kinase binding Biological Process: glycolysis |
| NCBI Summary: | The protein encoded by this gene is involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate, and a fructose-2,6-biphosphatase activity that catalyzes the degradation of fructose-2,6-bisphosphate. This protein regulates fructose-2,6-bisphosphate levels in the heart, while a related enzyme encoded by a different gene regulates fructose-2,6-bisphosphate levels in the liver and muscle. This enzyme functions as a homodimer. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O60825 |
| NCBI GenInfo Identifier: | 12643333 |
| NCBI Gene ID: | 5208 |
| NCBI Accession: | O60825.2 |
| UniProt Secondary Accession: | O60825,O60824, Q5VVQ3, Q5VVQ4, Q9H3P1, |
| UniProt Related Accession: | O60825 |
| Molecular Weight: | 54,406 Da |
| NCBI Full Name: | 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 |
| NCBI Synonym Full Names: | 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 |
| NCBI Official Symbol: | PFKFB2Â Â |
| NCBI Official Synonym Symbols: | PFK-2/FBPase-2Â Â |
| NCBI Protein Information: | 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 |
| UniProt Protein Name: | 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 |
| UniProt Synonym Protein Names: | 6PF-2-K/Fru-2,6-P2ase heart-type isozymeIncluding the following 2 domains:6-phosphofructo-2-kinase (EC:2.7.1.105)Fructose-2,6-bisphosphatase (EC:3.1.3.46) |
| Protein Family: | 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase |
| UniProt Gene Name: | PFKFB2Â Â |
| UniProt Entry Name: | F262_HUMAN |
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