The Phospho-PERK (T982) Polyclonal Antibody (CABP0886) is a valuable tool for researchers studying the PERK protein, a key player in the unfolded protein response pathway. This antibody, generated in rabbits, exhibits high specificity for human samples and is optimized for Western blotting applications. It recognizes the phosphorylated form of PERK at threonine 982, enabling precise detection and analysis in various cell types.PERK, also known as PKR-like endoplasmic reticulum kinase, is a crucial component of the unfolded protein response, which helps cells cope with endoplasmic reticulum stress and maintain protein homeostasis.
Dysregulation of PERK signaling has been implicated in various diseases, including neurodegenerative disorders, diabetes, and cancer. Therefore, studying the phosphorylation status of PERK can provide valuable insights into its role in disease progression and potentially uncover new therapeutic targets for intervention.
Antibody Name:
Anti-Phospho-PERK-T982 Antibody
Antibody SKU:
CABP0886
Antibody Size:
20uL, 50uL, 100uL
Application:
WB
Reactivity:
Human, Mouse, Rat
Host Species:
Rabbit
Immunogen:
A phospho specific peptide corresponding to residues surrounding T980 of human PERK.
Application:
WB
Recommended Dilution:
WB 1:500 - 1:2000
Reactivity:
Human, Mouse, Rat
Positive Samples:
293T
Immunogen:
A phospho specific peptide corresponding to residues surrounding T980 of human PERK.
Purification Method:
Affinity purification
Storage Buffer:
Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:
IgG
Sequence:
Email for sequence
Gene ID:
9451
Uniprot:
Q9NZJ5
Cellular Location:
Endoplasmic reticulum membrane, Single-pass type I membrane protein
Calculated MW:
125kDa
Observed MW:
170KDa
Western blot analysis of extracts of 293T cells, using Phospho-PERK-T982 Rabbit pAb (CABP0886) at 1:1000 dilution.293T cells were treated by IGF-1 (50 ng/ml) at 37'C for 5 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Synonyms:
EIF2AK3, PEK, PERK, WRS
Background:
The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. This protein is thought to modulate mitochondrial function. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome.
NCBI Summary:
The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. This protein is thought to modulate mitochondrial function. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome. [provided by RefSeq, Sep 2015]
