The Phospho-PEA15 (S104) Polyclonal Antibody (CABP0874) is a valuable tool for researchers studying the phosphorylation of PEA15 at serine 104. PEA15, also known as phosphoprotein enriched in astrocytes 15 kDa, is a protein involved in cell survival, proliferation, and apoptosis pathways. This antibody, raised in rabbits, is highly specific for detecting phosphorylated PEA15 at serine 104 in human samples, making it an essential tool for Western blot applications.Phosphorylation of PEA15 at serine 104 has been implicated in various signaling pathways, including the MAPK/ERK pathway, which is crucial for cell growth and survival. Research has shown that dysregulation of PEA15 phosphorylation can lead to abnormal cell proliferation and contribute to the development of cancer and other diseases.
By using this antibody to study the phosphorylation status of PEA15, researchers can gain valuable insights into the molecular mechanisms underlying these processes.Overall, the Phospho-PEA15 (S104) Polyclonal Antibody (CABP0874) is an essential tool for research in molecular biology, cell signaling, and cancer biology. Its high specificity and sensitivity make it ideal for studying the role of PEA15 phosphorylation in various cellular processes, paving the way for potential therapeutic interventions targeting these pathways.
Product Name:
Phospho-PEA15-S104 Rabbit Polyclonal Antibody
SKU:
CABP0874
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S104 of human PEA15 (NP_003759.1).
This gene encodes a death effector domain-containing protein that functions as a negative regulator of apoptosis. The encoded protein is an endogenous substrate for protein kinase C. This protein is also overexpressed in type 2 diabetes mellitus, where it may contribute to insulin resistance in glucose uptake. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
8682
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Phospho-PEA15-S104 Rabbit pAb (CABP0874) at 1:1000 dilution.C6 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
