The Phospho-PEA15 (S104) Polyclonal Antibody (CABP0788) is a valuable tool for researchers studying PEA15, a protein known for its involvement in cell survival, proliferation, and apoptosis. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the phosphorylated form of PEA15 at serine 104, this antibody enables precise detection and analysis of this key signaling molecule in various cell types.
PEA15 is a crucial player in multiple cellular processes, including the regulation of MAPK signaling pathways and the inhibition of apoptosis. Its dysregulation has been implicated in various diseases, making it a promising target for research in cancer, diabetes, and neurodegenerative disorders. By understanding the phosphorylation status of PEA15 at serine 104, researchers can gain insights into the mechanisms underlying these diseases and develop targeted therapies to treat them effectively.
Product Name:
Phospho-PEA15-S104 Rabbit Polyclonal Antibody
SKU:
CABP0788
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S104 of human PEA15 (NP_003759.1).
This gene encodes a death effector domain-containing protein that functions as a negative regulator of apoptosis. The encoded protein is an endogenous substrate for protein kinase C. This protein is also overexpressed in type 2 diabetes mellitus, where it may contribute to insulin resistance in glucose uptake. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
8682
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa and C6 cells, using Phospho-PEA15-S104 Rabbit pAb (CABP0788) at 1:2000 dilution. HeLa cells were treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight.C6 cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
