The Phospho-PDPK1 (S241) Polyclonal Antibody (CABP0426) is a valuable tool for researchers studying PDPK1, a protein kinase involved in various cellular processes, including cell growth, differentiation, and survival. This antibody, produced in rabbits, is highly specific for the phosphorylated form of PDPK1 at serine 241 and has been validated for use in Western blot applications.PDPK1, also known as 3-phosphoinositide-dependent protein kinase-1, is a key regulator of the PI3K/Akt signaling pathway, which is involved in cell proliferation and survival. Phosphorylation of PDPK1 at serine 241 is known to enhance its kinase activity and its ability to phosphorylate downstream targets, thereby regulating multiple cellular functions.
Research on PDPK1 and its phosphorylation at serine 241 is essential for understanding the mechanisms of cell signaling and how dysregulation of these pathways can lead to diseases such as cancer, diabetes, and cardiovascular disorders. By using this antibody to detect phospho-PDPK1 in various cell types, researchers can gain insights into the role of this protein in different cellular processes and potentially identify new therapeutic targets for disease intervention.
Product Name:
Phospho-PDPK1-S241 Rabbit Polyclonal Antibody
SKU:
CABP0426
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A phospho specific peptide corresponding to residues surrounding S241 of human PDK1/PDPK1
Enables 3-phosphoinositide-dependent protein kinase activity; phospholipase activator activity; and phospholipase binding activity. Involved in several processes, including cell surface receptor signaling pathway; regulation of protein kinase activity; and regulation of signal transduction. Acts upstream of or within intracellular signal transduction. Located in cell projection; cytosol; and plasma membrane. Implicated in prostate cancer. Biomarker of lung non-small cell carcinoma.
Purification Method:
Affinity purification
Gene ID:
5170
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of NIH/3T3, using Phospho-PDK1/PDPK1-S241 Rabbit pAb (CABP0426) at 1:1000 dilution.NIH/3T3 cells were treated by λ-PP mixed solution (1ul) at 30℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 30s.
