The Phospho-PDK1 (S241) Polyclonal Antibody (CABP0477) is designed for research involving Phospho-PDK1, a key regulatory protein involved in cell signaling pathways. The antibody, raised in rabbits, is highly specific and reactive towards human samples, making it ideal for Western blot applications. It targets the phosphorylated form of PDK1 at serine 241, allowing for precise detection and analysis in various cell types.Phosphorylation of PDK1 at serine 241 plays a crucial role in the activation of downstream signaling cascades involved in cell growth, proliferation, and metabolism.
Its dysregulation has been implicated in various diseases, including cancer, diabetes, and cardiovascular disorders. By understanding the activity and regulation of Phospho-PDK1, researchers can gain insights into disease mechanisms and potentially identify new therapeutic targets for intervention.Overall, the Phospho-PDK1 (S241) Polyclonal Antibody is a valuable tool for studying the role of PDK1 phosphorylation in cellular signaling pathways, making it an essential reagent for researchers in the fields of cell biology, biochemistry, and molecular medicine.
Product Name:
Phospho-PDPK1-S241 Rabbit Polyclonal Antibody
SKU:
CABP0477
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S241 of human PDK1/PDPK1 (NP_002604.1).
Enables 3-phosphoinositide-dependent protein kinase activity; phospholipase activator activity; and phospholipase binding activity. Involved in several processes, including cell surface receptor signaling pathway; regulation of protein kinase activity; and regulation of signal transduction. Acts upstream of or within intracellular signal transduction. Located in cell projection; cytosol; and plasma membrane. Implicated in prostate cancer. Biomarker of lung non-small cell carcinoma.
Purification Method:
Affinity purification
Gene ID:
5170
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of NIH/3T3 cells, using Phospho-PDK1/PDPK1-S241 pAb (CABP0477) at 1:1000 dilution.NIH/3T3 cells were treated by PDGF (100 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
