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Phospho-PDK1/PDPK1-S241 Monoclonal Antibody (CABP1357)

SKU:
CABP1357
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Applications:
WB
$167 - $731
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-PDK1/PDPK1-S241 Monoclonal Antibody
SKU:CABP1357
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:A synthetic phosphorylated peptide around S241 of human Phospho-PDK1/PDPK1-S241 (NP_002604.1).
Sequence:ANSF V
Tested Applications:WB ELISA
Recommended Dilution:WB,1:1000 - 1:5000
Synonyms:PDK1; PDPK2; PDPK2P; PRO0461; Phospho-PDK1/PDPK1-S241
Positive Sample:PC-3
Conjugate:Unconjugated
Cellular Localization:Cell junction, Cell membrane, Cytoplasm, Nucleus, Peripheral membrane protein, focal adhesion.
Calculated MW:63kDa
Observed MW:58-68kDa

Enables 3-phosphoinositide-dependent protein kinase activity; phospholipase activator activity; and phospholipase binding activity. Involved in several processes, including cell surface receptor signaling pathway; regulation of protein kinase activity; and regulation of signal transduction. Acts upstream of or within intracellular signal transduction. Located in cell projection; cytosol; and plasma membrane. Implicated in prostate cancer. Biomarker of lung non-small cell carcinoma.

Purification Method:Affinity purification
Gene ID:5170
Clone Number:ARC57184
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates, using Phospho-PDK1/PDPK1-S241 antibody (CABP1357) at1:2000 dilution.PC-3 cells were treated by λ-PP mixed solution (1ul) at 30℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 120s.

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