The Phospho-PDK1 (pPDPK1) S241 Monoclonal Antibody is a valuable tool for researchers studying the phosphorylation of PDK1 at serine 241, a key regulatory event in the activation of the PI3K/Akt signaling pathway. This antibody, produced using hybridoma technology, exhibits high specificity and sensitivity in detecting phosphorylated PDK1 in human samples and is suitable for use in various applications, including Western blotting.PDK1, a pivotal kinase in the PI3K signaling cascade, plays a crucial role in cell growth, survival, and metabolism. Phosphorylation at serine 241 is known to enhance PDK1 activity, leading to the phosphorylation and activation of downstream targets such as Akt.
Dysregulation of the PI3K/Akt pathway is implicated in various diseases, including cancer, diabetes, and cardiovascular disorders, making the study of PDK1 phosphorylation essential for understanding disease mechanisms and identifying therapeutic targets.By using the Phospho-PDK1 S241 Monoclonal Antibody, researchers can investigate the dynamic regulation of PDK1 phosphorylation and its implications in cellular signaling pathways. This antibody provides a reliable tool for exploring the role of PDK1 in disease progression and may offer new insights into potential therapeutic strategies targeting the PI3K/Akt pathway.
Product Name:
Phospho-PDK1/PDPK1-S241 Monoclonal Antibody
SKU:
CABP1357
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around S241 of human Phospho-PDK1/PDPK1-S241 (NP_002604.1).
Enables 3-phosphoinositide-dependent protein kinase activity; phospholipase activator activity; and phospholipase binding activity. Involved in several processes, including cell surface receptor signaling pathway; regulation of protein kinase activity; and regulation of signal transduction. Acts upstream of or within intracellular signal transduction. Located in cell projection; cytosol; and plasma membrane. Implicated in prostate cancer. Biomarker of lung non-small cell carcinoma.
Purification Method:
Affinity purification
Gene ID:
5170
Clone Number:
ARC57184
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using Phospho-PDK1/PDPK1-S241 antibody (CABP1357) at1:2000 dilution.PC-3 cells were treated by λ-PP mixed solution (1ul) at 30℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 120s.
