The Phospho-PDHA1 (S293) Rabbit Monoclonal Antibody (CABP1022) is specifically designed for researchers studying the phosphorylation of PDHA1, a key enzyme in the regulation of glucose metabolism and energy production. This antibody, generated in rabbits, exhibits high specificity for human samples and has been validated for use in Western blot applications.PDHA1 phosphorylation at serine 293 plays a crucial role in the regulation of its enzymatic activity, impacting cellular energy metabolism and oxidative stress response. The Phospho-PDHA1 (S293) antibody allows for the detection and quantification of phosphorylated PDHA1, providing valuable insights into the metabolic processes and signaling pathways involved in cell function.
Research involving PDHA1 phosphorylation is essential for understanding how alterations in glucose metabolism contribute to disease states such as cancer, diabetes, and neurodegenerative disorders. By studying the phosphorylation status of PDHA1, researchers can uncover potential therapeutic targets and develop strategies to manipulate metabolic pathways for therapeutic purposes.
Product Name:
Phospho-PDHA1-S293 Rabbit Monoclonal Antibody
SKU:
CABP1022
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S293 of human PDHA1 (P08559).
Sequence:
GHSM SD
Tested Applications:
WBIPELISA
Recommended Dilution:
WB,1:2000 - 1:20000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
5160
Clone Number:
ARC53489
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-PDHA1-S293 Rabbit mAb (CABP1022) at 1:23000 dilution.293T cells were treated by λ-PP mixed solution (1ul) at 30℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
