The Phospho-PDGFRB (Y771) Polyclonal Antibody (CABP1062) is a powerful tool for researchers studying the phosphorylation of PDGFRB at tyrosine 771, a crucial event in signal transduction pathways involved in cell growth and differentiation. This antibody, raised in rabbits, exhibits high specificity and sensitivity towards human samples, making it an excellent choice for Western blot analyses. By targeting the phosphorylated form of PDGFRB, researchers can gain valuable insights into the regulation of cellular processes related to development, wound healing, and cancer progression.PDGFRB, a member of the receptor tyrosine kinase family, is known to play a key role in various cellular functions, including cell proliferation, migration, and survival.
Phosphorylation of PDGFRB at tyrosine 771 triggers downstream signaling cascades that ultimately impact cell behavior and tissue remodeling. Therefore, understanding the dynamics of PDGFRB phosphorylation is essential for unraveling the mechanisms underlying diseases such as cancer, fibrosis, and vascular disorders.Overall, the Phospho-PDGFRB (Y771) Polyclonal Antibody (CABP1062) offers researchers a reliable tool for investigating the role of PDGFRB phosphorylation in physiological and pathological conditions. Its specificity and performance make it an indispensable asset for studies focused on dissecting signal transduction pathways and identifying new therapeutic targets in various disease contexts.
Product Name:
Phospho-PDGFRB-Y771 Rabbit Polyclonal Antibody
SKU:
CABP1062
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around Y771 of human PDGFRB (NP_002600.1).
Cell membrane, Cytoplasmic vesicle, Lysosome lumen, Single-pass type I membrane protein.
Calculated MW:
124kDa
Observed MW:
190kDa
The protein encoded by this gene is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer (PDGFB or PDGFD) or a heterodimer (PDGFA and PDGFB). This gene is essential for normal development of the cardiovascular system and aids in rearrangement of the actin cytoskeleton. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the ETV6 gene, results in chronic myeloproliferative disorder with eosinophilia.
Purification Method:
Affinity purification
Gene ID:
5159
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-PDGFRB-Y771 pAb (CABP1062) at 1:1000 dilution or Phospho-PDGFRB-Y771 antibody (CAB2180).NIH/3T3 cells were treated by PDGF (100 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
