The Phospho-PDGFRα (Y849) Polyclonal Antibody (CABP0569) is specifically designed for research involving the phosphorylated form of PDGFRα at tyrosine 849, a crucial signaling molecule involved in cell growth and differentiation. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in applications such as Western blotting.The phosphorylation of PDGFRα at tyrosine 849 plays a key role in the activation of downstream signaling pathways involved in cell proliferation, migration, and survival. Research into the regulation of this phosphorylation event is important in understanding the mechanisms underlying diseases such as cancer, where dysregulation of PDGFRα signaling can drive tumor growth and metastasis.
By using the Phospho-PDGFRα (Y849) Polyclonal Antibody, researchers can detect and analyze the phosphorylation status of PDGFRα in various cell types, providing valuable insights into its role in physiological and pathological processes. This antibody is a valuable tool for studying signaling pathways related to cell growth and differentiation, making it ideal for investigations in cancer biology and drug development.
Product Name:
Phospho-PDGFRA-Y849 Rabbit Polyclonal Antibody
SKU:
CABP0569
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around Y849 of human PDGFRA (NP_006197.1).
Sequence:
SNYV S
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
CD140A; PDGFR2; PDGFR-2; Phospho-PDGFR alpha-Y849
Positive Sample:
HepG2
Conjugate:
Unconjugated
Cellular Localization:
Cell membrane, Single-pass type I membrane protein.
Calculated MW:
123kDa
Observed MW:
190kDa
This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies suggest that this gene plays a role in organ development, wound healing, and tumor progression. Mutations in this gene have been associated with idiopathic hypereosinophilic syndrome, somatic and familial gastrointestinal stromal tumors, and a variety of other cancers.
Purification Method:
Affinity purification
Gene ID:
5156
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from HepG2 cells, using Phospho-PDGFR alpha-Y849 Rabbit pAb (CABP0569) at 1:1000 dilution. HepG2 cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.
