The Phospho-PDGFRα (Y754) Polyclonal Antibody (CABP0568) is a valuable tool for researchers studying the PDGFRα protein, a key player in cell growth and development. This antibody, raised in rabbits, is highly specific to the phosphorylated form of PDGFRα at the Y754 residue and is validated for use in Western blot applications.PDGFRα is a receptor tyrosine kinase that plays a crucial role in cell signaling pathways that regulate cell proliferation, migration, and survival. Phosphorylation of PDGFRα at the Y754 residue is known to be important for its activation and downstream signaling cascades. By targeting this phosphorylation site, researchers can gain insights into the mechanisms underlying PDGFRα activation and its impact on cellular processes.
This antibody is ideal for studies in cancer biology, as dysregulation of PDGFRα signaling is commonly associated with various types of cancer. By investigating the phosphorylation status of PDGFRα at the Y754 residue, researchers can uncover potential therapeutic targets for cancer treatment and drug development.Overall, the Phospho-PDGFRα (Y754) Polyclonal Antibody is a valuable tool for researchers interested in exploring the role of PDGFRα in cell signaling pathways, cancer biology, and other areas of biomedical research. Its specificity and reliability make it a trusted reagent for Western blot analysis and furthering our understanding of PDGFRα
Product Name:
Phospho-PDGFRA-Y754 Rabbit Polyclonal Antibody
SKU:
CABP0568
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around Y754 of human PDGFRA (NP_006197.1).
Sequence:
SKYS D
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
CD140A; PDGFR2; PDGFR-2; Phospho-PDGFR alpha-Y754
Positive Sample:
HepG2
Conjugate:
Unconjugated
Cellular Localization:
Cell membrane, Single-pass type I membrane protein.
Calculated MW:
123kDa
Observed MW:
180kDa
This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies suggest that this gene plays a role in organ development, wound healing, and tumor progression. Mutations in this gene have been associated with idiopathic hypereosinophilic syndrome, somatic and familial gastrointestinal stromal tumors, and a variety of other cancers.
Purification Method:
Affinity purification
Gene ID:
5156
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from HepG2 cells, using Phospho-PDGFR alpha-Y754 Rabbit pAb (CABP0568) at 1:1000 dilution. HepG2 cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.
