The KO Validated SPK Rabbit Polyclonal Antibody (CAB19947) is a powerful tool for researchers studying SPK, a key protein involved in signaling pathways related to inflammation and immune responses. This antibody, generated in rabbits, shows high reactivity with samples from knockout models, ensuring specificity and reliability in experimental settings.SPK, also known as Signal Transducing Protein Kinase, plays a critical role in cell signaling cascades that regulate immune responses and inflammatory processes. By targeting SPK with this validated antibody, researchers can accurately detect and analyze protein expression levels in various cell types, providing valuable insights into the mechanisms underlying immune regulation and inflammatory diseases.
The KO Validated SPK Rabbit Polyclonal Antibody is specifically validated for use in Western blot applications, offering researchers a precise and efficient method for studying SPK expression levels and activity. With its high specificity and sensitivity, this antibody is an essential tool for investigations in immunology, cancer research, and other fields where SPK signaling pathways play a significant role.
Product Name:
[KO Validated] SPK Rabbit Polyclonal Antibody
SKU:
CAB19947
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-322 of human SPK (NP_060962.2).
This gene encodes a serine/threonine protein kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. The encoded protein may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. Overexpression of this gene has been implicated in tumorigenesis. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
55872
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and SPK knockout (KO) 293T cells, using [KO Validated] SPK Rabbit pAb (CAB19947) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
