Phospho-Paxillin-Y118 Antibody (CABP0156)
- SKU:
- CABP0156
- Product Type:
- Antibody
- Applications:
- WB
- IHC
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
Anti-Phospho-Paxillin-Y118 Antibody (CABP0156)
The Phospho-Paxillin (Y118) Polyclonal Antibody (CABP0156) is a valuable tool for researchers studying signaling pathways involved in cell adhesion and migration. Paxillin is a focal adhesion adaptor protein that plays a crucial role in linking integrin signaling to actin cytoskeleton dynamics. This antibody, raised in rabbits, specifically targets phospho-Paxillin at tyrosine 118, a critical phosphorylation site involved in regulating the function of Paxillin.The Phospho-Paxillin (Y118) Polyclonal Antibody is highly reactive with human samples and has been validated for use in Western blot applications. By targeting the phosphorylated form of Paxillin, researchers can investigate the activation status of Paxillin in response to various stimuli and conditions. This antibody enables the detection and analysis of phospho-Paxillin in different cell types, allowing for a better understanding of its role in cell migration, invasion, and adhesion processes.
Studies have shown that phosphorylation of Paxillin at Y118 is associated with increased cell motility and invasion in cancer cells, suggesting its potential importance in cancer progression and metastasis. Additionally, dysregulation of Paxillin signaling has been implicated in various other diseases, including cardiovascular disorders and inflammatory conditions. Understanding the regulatory mechanisms of phospho-Paxillin Y118 is essential for developing targeted therapies that can modulate cell adhesion and migration in disease states.Overall, the Phospho-Paxillin (
| Antibody Name: | Anti-Phospho-Paxillin-Y118 Antibody |
| Antibody SKU: | CABP0156 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding Y118 of human PXN |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, 3T3 |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding Y118 of human PXN |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 5829 |
| Uniprot: | P49023 |
| Cellular Location: | Cell junction, Cytoplasm, cell cortex, cytoskeleton, focal adhesion |
| Calculated MW: | 46kDa/60kDa/64kDa/66kDa |
| Observed MW: | 70kDa |
 | Western blot analysis of extracts from 3T3 cells, using phospho-PXN-Y118 antibody (CABP0156). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. |
 | Western blot analysis of extracts of HeLa cells, using Phospho-PXN-Y118 antibody (CABP0156) at 1:2000 dilution. HeLa cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded human breast carcinoma tissue, using Phospho-PXN-Y118 antibody (CABP0156). |
| Synonyms: | Paxillin, PXN, paxillin |
| Background: | This gene encodes a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties (PMID:9054445). |
| UniProt Protein Function: | PXN: a multi-domain cytoskeletal protein involved in actin-membrane attachment that localizes primarily to focal adhesion sites to the extracellular matrix. Phosphorylated by focal adhesion kinase (FAK) and is a component of integrin signaling. Its phosphorylation provides docking sites for recruitment of signaling molecules to focal adhesions. Binds in vitro to vinculin as well as to the SH3 domain of c-SRC and, when tyrosine phosphorylated, to the Crk SH2 domain. Interacts with GIT1, NUDT16L1/SDOS, PARVA and TGFB1I1. Component of cytoplasmic complexes, which also contain GIT1, ARHGEF6 and PAK1. Binds DDEF2. Interacts with unphosphorylated ITGA4. Interacts with RNF5.Three alternatively-spliced isoforms have been described. Isoform beta binds to focal adhesion kinase but weakly to vinculin. Isoform gamma binds to vinculin but only weakly to focal adhesion kinase. |
| UniProt Protein Details: | Protein type:Adaptor/scaffold; Cytoskeletal; Motility/polarity/chemotaxis; Cell adhesion Chromosomal Location of Human Ortholog: 12q24.31 Cellular Component: nucleoplasm; microtubule associated complex; focal adhesion; lamellipodium; cytoplasm; stress fiber; plasma membrane; cell cortex; cytosol Molecular Function:integrin binding; protein binding; zinc ion binding; beta-catenin binding; BH4 domain binding; protein kinase binding; vinculin binding Biological Process: integrin-mediated signaling pathway; lamellipodium biogenesis; epidermal growth factor receptor signaling pathway; focal adhesion formation; regulation of cell shape; peptidyl-tyrosine phosphorylation; branching morphogenesis of a tube; muscle contraction; activation of MAPK activity; transforming growth factor beta receptor signaling pathway; signal complex assembly; cytoskeleton organization and biogenesis; vascular endothelial growth factor receptor signaling pathway; signal transduction; cell adhesion |
| NCBI Summary: | This gene encodes a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties (PMID:9054445). [provided by RefSeq, Aug 2011] |
| UniProt Code: | P49023 |
| NCBI GenInfo Identifier: | 317373486 |
| NCBI Gene ID: | 5829 |
| NCBI Accession: | P49023.3 |
| UniProt Secondary Accession: | P49023,O14970, O14971, O60360, Q5HYA4, B2RAI3, B7ZMB4 |
| UniProt Related Accession: | P49023 |
| Molecular Weight: | 68kDa |
| NCBI Full Name: | Paxillin |
| NCBI Synonym Full Names: | paxillin |
| NCBI Official Symbol: | PXNÂ Â |
| NCBI Protein Information: | paxillin |
| UniProt Protein Name: | Paxillin |
| Protein Family: | Paxillin |
| UniProt Gene Name: | PXNÂ Â |
| UniProt Entry Name: | PAXI_HUMAN |
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