The Phospho-PAK4/PAK5/PAK6 (S474/S560/S602) Rabbit Monoclonal Antibody (CABP1049) is a powerful tool for researchers studying the PAK family of proteins, specifically focusing on phosphorylation at serine residues 474, 560, and 602. This antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications.The PAK family of proteins are key regulators of cell proliferation, survival, and cytoskeletal organization, with dysregulation implicated in various diseases including cancer. Phosphorylation of PAK4, PAK5, and PAK6 at specific residues like S474, S560, and S602 has been shown to impact their functions, highlighting the importance of studying these modifications in cell signaling pathways.
By using this monoclonal antibody, researchers can detect and assess the phosphorylation status of PAK4, PAK5, and PAK6 in various cell types, providing valuable insights into their roles in physiological and pathological processes. This antibody is an essential tool for studies in cancer biology, intracellular signaling, and drug development targeting the PAK family of proteins.
PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Purification Method:
Affinity purification
Gene ID:
10298/57144/56924
Clone Number:
ARC1596
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Phospho-PAK4/PAK5/PAK6-S474/S560/S602 Rabbit mAb (CABP1049) at 1:1000 dilution.Both NIH/3T3 cells and C6 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
