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Phospho-PAK4/PAK5/PAK6-S474/S560/S602 Rabbit Monoclonal Antibody (CABP1049)

SKU:
CABP1049
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Monoclonal Antibody
€419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-PAK4/PAK5/PAK6-S474/S560/S602 Rabbit Monoclonal Antibody
SKU:CABP1049
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Mouse,Rat
Immunogen:A synthetic phosphorylated peptide around S474 of human PAK4PAK4 (O96013).
Sequence:RKSL V
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:PAK4; KIAA1142; PAK5KIAA1264; PAK6; Phospho-PAK4/PAK5/PAK6-S474/S560/S602
Positive Sample:NIH/3T3 PMA,C6 PMA
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Nucleus
Calculated MW:64, 80, 75 kDa
Observed MW:64kDa,75kDa,70kDa

PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Purification Method:Affinity purification
Gene ID:10298/57144/56924
Clone Number:ARC1596
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of various cell lines, using Phospho-PAK4/PAK5/PAK6-S474/S560/S602 Rabbit mAb (CABP1049) at 1:1000 dilution.Both NIH/3T3 cells and C6 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

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