The Phospho-Pak2(S20) Polyclonal Antibody (CABP0803) is a powerful tool for researchers studying the Pak2 protein, a key regulator of cell proliferation, survival, and migration. This antibody, generated in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. It recognizes the phosphorylated form of Pak2 at serine 20, allowing for precise detection and analysis in a variety of cell types.Pak2 is a serine/threonine kinase that is involved in signaling pathways regulating cell growth and survival. Phosphorylation of Pak2 at serine 20 is known to enhance its kinase activity, influencing downstream cellular processes.
The Phospho-Pak2(S20) Polyclonal Antibody is an essential tool for researchers studying the role of Pak2 in cancer progression, metastasis, and drug resistance.Understanding the phosphorylation status of Pak2 is crucial for unraveling its function in cancer biology and developing targeted therapies. By utilizing the Phospho-Pak2(S20) Polyclonal Antibody, researchers can gain valuable insights into the signaling pathways regulated by Pak2 and its potential as a therapeutic target in cancer treatment.
Product Name:
Phospho-PAK2-S20 Rabbit Polyclonal Antibody
SKU:
CABP0803
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S20 of human PAK2 (NP_002568.2).
The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The protein encoded by this gene is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell.
Purification Method:
Affinity purification
Gene ID:
5062
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from NIH/3T3 cells using Phospho-PAK2-S20 Rabbit pAb (CABP0803) at 1:1000 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection:ECL Basic Kit (AbGn00020).Exposuretime: 30s.
