The Phospho-PAK2 (S20) Monoclonal Antibody is a valuable tool for researchers studying the PAK2 protein, which is involved in cell signaling pathways related to cell proliferation, survival, and migration. The antibody is designed to specifically target and detect phosphorylated PAK2 at serine 20, a critical site for regulating its activity.Raised in mice, this antibody is highly sensitive and specific for detecting phosphorylated PAK2 in human samples, making it a reliable tool for Western blot and immunofluorescence applications. By targeting the phosphorylated form of PAK2, researchers can gain insights into the activation status of this important signaling protein in various cell types and conditions.The phosphorylation of PAK2 at serine 20 has been linked to its role in regulating cytoskeletal dynamics, cell motility, and cell cycle progression.
Studies have shown that dysregulation of PAK2 activity, including abnormal phosphorylation patterns, is associated with various diseases, including cancer and neurological disorders. Therefore, understanding the phosphorylation status of PAK2 is essential for unraveling its complex functions and potential therapeutic implications.Overall, the Phospho-PAK2 (S20) Monoclonal Antibody offers researchers a reliable tool for investigating the role of phosphorylated PAK2 in cell signaling pathways, making it a valuable asset for studies in cancer biology, cell biology, and drug development research.
Product Name:
Phospho-PAK2-S20 Monoclonal Antibody
SKU:
CABP1443
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S20 of human PAK2 (NP_002568.2).
Sequence:
MSST I
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:1000 - 1:5000
Synonyms:
KNO2; PAK65; PAKgamma; Phospho-PAK2-S20
Positive Sample:
NIH/3T3+CA,C6+CA
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Membrane, Nucleus
Calculated MW:
58kDa
Observed MW:
61kDa/67kDa
The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The protein encoded by this gene is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell.
Purification Method:
Affinity purification
Gene ID:
5062
Clone Number:
ARC62567
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of NIH/3T3, using Phospho-PAK2-S20 Rabbit mAb (CABP1443) at 1:2000 dilution.NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
