The Anti-PAK1/PAK2/PAK3 Antibody (CAB21090) is a powerful tool for researchers studying the P21-activated kinase (PAK) family of proteins. These serine/threonine kinases play important roles in cell signaling, cytoskeletal dynamics, and cell proliferation. The antibody, generated in rabbits, exhibits high specificity and sensitivity towards PAK1, PAK2, and PAK3 proteins in human samples, making it ideal for Western blotting and immunohistochemistry applications.PAK proteins are involved in various cellular processes, including cell motility, cell cycle progression, and gene transcription. Dysregulation of PAK signaling has been implicated in various diseases, such as cancer, neurological disorders, and infectious diseases.
By targeting PAK1, PAK2, and PAK3, researchers can gain insights into the molecular mechanisms underlying these pathologies and potentially identify novel therapeutic targets.Overall, the Anti-PAK1/PAK2/PAK3 Antibody (CAB21090) offers researchers a valuable tool for investigating the functions and dysregulation of PAK proteins in health and disease. Its high specificity and sensitivity make it a reliable reagent for studying PAK signaling pathways and developing potential interventions for PAK-related disorders.
Product Name:
PAK1/PAK2/PAK3 Polyclonal Antibody
SKU:
CAB21090
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 350-450 of human PAK1/PAK2/PAK3 (NP_002567.3).
cytoplasm, cytosol, focal adhesion, microtubule organizing center, nuclear membrane, nucleoplasm, plasma membrane, ruffle membrane, Z disc
Calculated MW:
65kDa
Observed MW:
61kDa, 66-68kDa
This gene encodes a family member of serine/threonine p21-activating kinases, known as PAK proteins. These proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling, and they serve as targets for the small GTP binding proteins Cdc42 and Rac. This specific family member regulates cell motility and morphology. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2010]
Purification Method:
Affinity purification
Gene ID:
5058/5062/5063
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PAK1/PAK2/PAK3 antibody (CAB21090) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
