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Phospho-p90Rsk/RSK1/RPS6KA1-S380 Rabbit Polyclonal Antibody (CABP0562)

SKU:
CABP0562
Product Type:
Antibody
Applications:
WB
IP
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Cycle
  • Cell Cycle Antibodies 2 Anti-Phospho-P90RSK-S380 Antibody CABP0562
  • Cell Cycle Antibodies 2 Anti-Phospho-P90RSK-S380 Antibody CABP0562
  • Cell Cycle Antibodies 2 Anti-Phospho-P90RSK-S380 Antibody CABP0562
$503
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Description

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Product Name:Phospho-p90Rsk/RSK1/RPS6KA1-S380 Rabbit Polyclonal Antibody
SKU:CABP0562
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic phosphorylated peptide around S380 of human p90Rsk/RSK1/RPS6KA1 (NP_002944.2).
Sequence:GFSF V
Tested Applications:WB IP ELISA
Recommended Dilution:WB,1:500 - 1:2000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:RSK; HU-1; RSK1; p90Rsk; MAPKAPK1; MAPKAPK1A; Phospho-p90Rsk/RSK1/RPS6KA1-S380
Positive Sample:HeLa,NIH/3T3
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Nucleus.
Calculated MW:83kDa
Observed MW:90kDa

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Purification Method:Affinity purification
Gene ID:6195
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using Phospho-p90Rsk/RSK1/RPS6KA1-S380 Rabbit pAb (CABP0562) at 1:1000 dilution or RPS6KA1 antibody (CAB15718). HeLa cells were treated by PMA/TPA (200 nM) at 37℃ for 15 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

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