The Phospho-p90RSK (S380) Polyclonal Antibody (CABP0562) is specifically designed for research involving the phosphorylated form of the p90 ribosomal S6 kinase (p90RSK) at serine 380. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By targeting the phosphorylated form of p90RSK, this antibody allows for the detection and analysis of p90RSK activation in various cell types.The p90RSK protein plays a crucial role in cell growth, proliferation, and survival through its involvement in various signaling pathways. Phosphorylation of p90RSK at serine 380 is known to activate its kinase activity, leading to downstream effects on gene expression and cell function.
Research into the phosphorylation status of p90RSK can provide insights into cellular signaling pathways involved in diseases such as cancer, neurodegenerative disorders, and inflammatory conditions.Understanding the activation of p90RSK through phosphorylation at serine 380 is essential for elucidating the molecular mechanisms underlying cell signaling and its implications in disease pathology. The Phospho-p90RSK (S380) Polyclonal Antibody (CABP0562) offers a valuable tool for researchers studying the role of p90RSK in cellular processes and disease development.
This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Purification Method:
Affinity purification
Gene ID:
6195
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using Phospho-p90Rsk/RSK1/RPS6KA1-S380 Rabbit pAb (CABP0562) at 1:1000 dilution or RPS6KA1 antibody (CAB15718). HeLa cells were treated by PMA/TPA (200 nM) at 37℃ for 15 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
