The p90Rsk/RSK1/RPS6KA1 Antibody (CAB15718) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, demonstrates high specificity for human samples and is suitable for use in Western blot applications.p90RSK is a serine/threonine kinase that is activated by various growth factors and cellular stress signals. Its involvement in signaling pathways related to cell growth and survival makes it an important target for research in cancer biology and developmental biology. By detecting and analyzing the p90RSK protein in different cell types, this antibody can provide valuable insights into the mechanisms underlying cell signaling and growth regulation.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
p90Rsk/RSK1/RPS6KA1 Antibody
SKU:
CAB15718
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Purification Method
Affinity purification
Gene ID
6195
RRID
AB_2763131
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using p90Rsk/RSK1/RPS6KA1 Rabbit pAb (CAB15718) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Mouse spleen using p90Rsk/RSK1/RPS6KA1 Rabbit pAb (CAB15718) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunofluorescence analysis of U-2 OS cells using p90Rsk/RSK1/RPS6KA1 Rabbit pAb (CAB15718) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
