The Phospho-p90RSK (S363) Polyclonal Antibody (CABP0767) is a valuable tool for researchers studying the phosphorylation of p90RSK at serine 363, a key post-translational modification that regulates the activity of this protein kinase. The antibody, raised in rabbits, exhibits high specificity and sensitivity for detecting phosphorylated p90RSK S363 in human samples, making it suitable for Western blot analysis.p90RSK is a serine/threonine kinase that plays a crucial role in various cellular processes, including cell growth, survival, and proliferation. Phosphorylation of p90RSK at S363 is known to activate its kinase activity, leading to the phosphorylation of downstream targets involved in signal transduction pathways.
Understanding the regulation of p90RSK phosphorylation at S363 is essential for elucidating its role in cancer, development, and other biological processes.By using the Phospho-p90RSK (S363) Polyclonal Antibody (CABP0767), researchers can gain valuable insights into the mechanisms governing p90RSK activation and its impact on cell signaling pathways. This antibody is a valuable tool for studies in cancer biology, cell signaling, and drug development, offering important implications for therapeutic interventions targeting the p90RSK pathway.
This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Purification Method:
Affinity purification
Gene ID:
6195
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using (CABP0767) at 1:1000 dilution.HeLa cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
