The Phospho-P70S6K1 (T389) Polyclonal Antibody (CABP0564) is a powerful tool for researchers studying the phosphorylation of p70S6K1, a key regulator of cell growth and protein synthesis. This antibody, produced in rabbits, shows high reactivity with human samples and is validated for use in Western blotting applications.P70S6K1 is a serine/threonine kinase that is involved in multiple cellular processes, including cell proliferation, survival, and metabolism. Phosphorylation of p70S6K1 at threonine 389 is a crucial event that activates its kinase activity and promotes downstream signaling pathways. This antibody specifically recognizes the phosphorylated form of p70S6K1 at threonine 389, enabling researchers to investigate the regulation and function of this important signaling molecule.
With its ability to detect and quantify phospho-p70S6K1 (T389) in various cell types, this antibody is ideal for studies investigating cell signaling pathways, growth factor signaling, and cancer biology. By targeting this critical phosphorylation event, researchers can gain valuable insights into the role of p70S6K1 in cellular processes and disease development, paving the way for the development of novel therapeutic strategies targeting this pathway.
This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17.
Purification Method:
Affinity purification
Gene ID:
6198
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-p70 S6 Kinase 1-T389 Rabbit pAb (CABP0564) at 1:1000 dilution.NIH/3T3 cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
