The Phospho-p70S6K1 (S371) Polyclonal Antibody (CABP0482) is an essential tool for researchers studying the phosphorylation status of p70S6K1, a key protein involved in the regulation of cell growth and protein synthesis. This antibody, produced in rabbits, is highly specific for detecting phosphorylation at serine 371 of p70S6K1 in human samples and is validated for use in Western blot applications.Phosphorylation of p70S6K1 at serine 371 plays a crucial role in the activation of this protein, leading to downstream signaling that promotes cell proliferation and survival. By targeting this specific phosphorylation site, researchers can gain insights into the signaling pathways that regulate cellular processes such as growth and metabolism.
The Phospho-p70S6K1 (S371) Polyclonal Antibody is a valuable tool for studies in molecular biology, cell signaling, and cancer research, providing researchers with the ability to investigate the role of p70S6K1 phosphorylation in various cellular processes and disease states. This antibody's high specificity and sensitivity make it an ideal choice for studying the intricate regulatory mechanisms that govern cellular growth and proliferation.
Product Name:
Phospho-p70 S6 Kinase 1-S371 Polyclonal Antibody
SKU:
CABP0482
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse
Immunogen:
A synthetic phosphorylated peptide around S371 of human P70S6K1 (NP_001258989.1).
This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17.
Purification Method:
Affinity purification
Gene ID:
6198
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of NIH/3T3, using Phospho-p70 S6 Kinase 1-S371 antibody (CABP0482) at 1:400 dilution.NIH/3T3 cells were treated by EGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
