The Phospho-p70 S6 Kinase (T421/S424) Polyclonal Antibody (CABP1106) is specifically designed for research involving the phosphorylation of p70 S6 kinase, a key signaling molecule involved in the regulation of cell growth and protein synthesis. This rabbit polyclonal antibody is highly reactive with samples from multiple species, including human, mouse, and rat, and has been validated for use in Western blot and immunohistochemistry applications.The antibody binds to the phosphorylated form of p70 S6 kinase at threonine 421 and serine 424, allowing for precise detection and analysis of this critical signaling pathway component in various cell types. It is an essential tool for studying the regulation of cell proliferation, differentiation, and survival, making it particularly useful for research in cancer biology and drug development.
The phosphorylation of p70 S6 kinase at T421/S424 is a crucial event in the activation of this enzyme, leading to downstream signaling cascades that control protein synthesis and cell growth. Understanding the role of this phosphorylation event is essential for unraveling the mechanisms underlying cell growth regulation and identifying potential therapeutic targets for diseases such as cancer and metabolic disorders. The CABP1106 antibody provides researchers with a powerful tool for investigating the functions of p70 S6 kinase in normal and disease contexts, furthering our understanding of cellular signaling pathways and contributing to the development of novel treatment strategies.
This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17.
Purification Method:
Affinity purification
Gene ID:
6198
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-p70 S6 Kinase 1-T421/S424 pAb (CABP1106) at 1:1000 dilution or P70 S6K antibody (CAB16968).NIH/3T3 cells were treated by EGF (100 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
