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Phospho-p70 S6 Kinase 1-T421/S424 Rabbit Monoclonal Antibody (CABP0502)

SKU:
CABP0502
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Monoclonal Antibody
Research Area:
Cell Death
$503
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Description

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Product Name:Phospho-p70 S6 Kinase 1-T421/S424 Rabbit Monoclonal Antibody
SKU:CABP0502
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthesized peptide derived from human Phospho-S6K1 (T421 + S424).(NP_001258989.1)
Sequence:RRFI GSPR TPVS PVKF SPGD
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200
Synonyms:S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha; Phospho-p70 S6 Kinase 1-T421/S424
Positive Sample:NIH/3T3+serum,C6+serum
Conjugate:Unconjugated
Cellular Localization:Cell junction, Cytoplasm, Mitochondrion outer membrane, Mitochondrion, Nucleus, synapse, synaptosome.
Calculated MW:59kDa
Observed MW:65kDa

This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17.

Purification Method:Affinity purification
Gene ID:6198
Clone Number:ARC0107
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using Phospho-p70 S6 Kinase 1-T421/S424 Rabbit mAb (CABP0502) at 1:1000 dilution.NIH/3T3 and C6 cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnightSecondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60S.

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