The Phospho-p70 S6 Kinase (T421/S424) Rabbit Monoclonal Antibody (CABP0502) is a valuable tool for research involving the p70 S6 Kinase signaling pathway, which plays a crucial role in cell growth and proliferation. The antibody, developed using rabbits, shows high reactivity with phosphorylated p70 S6 Kinase at threonine 421 and serine 424 residues in human samples, making it ideal for Western blot applications.p70 S6 Kinase is a key regulator of protein synthesis and cell survival, making it a promising target for cancer research and studies on cell signaling pathways. The phosphorylation of p70 S6 Kinase at T421/S424 is known to activate its kinase activity, leading to downstream effects on protein translation and cell growth.
By using the Phospho-p70 S6 Kinase (T421/S424) Rabbit Monoclonal Antibody, researchers can effectively study the activation and regulation of the p70 S6 Kinase pathway in different cell types and experimental settings. This antibody is a valuable tool for investigating the role of p70 S6 Kinase in various physiological and pathological conditions, providing insights that could contribute to the development of novel therapeutic strategies targeting this signaling pathway.
This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17.
Purification Method:
Affinity purification
Gene ID:
6198
Clone Number:
ARC0107
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using Phospho-p70 S6 Kinase 1-T421/S424 Rabbit mAb (CABP0502) at 1:1000 dilution.NIH/3T3 and C6 cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnightSecondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60S.
