The Phospho-p70 S6 Kinase (T389) Rabbit Polyclonal Antibody (CABP1059) is specifically designed for research involving the phosphorylated form of p70 S6 kinase, an important signaling protein involved in cell growth and proliferation. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.p70 S6 kinase is a key regulator of protein synthesis and cell growth, making it a crucial target for studies in cancer biology, cell signaling, and metabolic regulation.
The phosphorylation of p70 S6 kinase at threonine 389 is a critical event in its activation and is often used as a marker for mTOR pathway activity.By using the Phospho-p70 S6 Kinase (T389) Rabbit Polyclonal Antibody, researchers can accurately detect and measure the phosphorylated form of p70 S6 kinase in various cell types and experimental conditions. This antibody is an essential tool for investigating the role of p70 S6 kinase in cellular processes and for identifying potential therapeutic targets in cancer and other diseases.
This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17.
Purification Method:
Affinity purification
Gene ID:
6198
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-p70 S6 Kinase 1-T389 Rabbit pAb (CABP1059) at 1:500 dilution.NIH/3T3 cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
