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Phospho-p70 S6 kinase-T389 Monoclonal Antibody (CABP1389)

SKU:
CABP1389
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Applications:
WB
$167 - $731
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-p70 S6 kinase-T389 Monoclonal Antibody
SKU:CABP1389
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Mouse,Rat
Immunogen:A synthetic phosphorylated peptide around T389 of human p70 S6 kinase(NP_003152.1).
Sequence:LGFT YVA
Tested Applications:WB ELISA
Recommended Dilution:WB,1:2000 - 1:9000
Synonyms:S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha; Phospho-p70 S6 kinase-T389
Positive Sample:NIH/3T3+FBS,C6
Conjugate:Unconjugated
Cellular Localization:Cell junction, Cytoplasm, Mitochondrion outer membrane, Mitochondrion, Nucleus, synapse, synaptosome.
Calculated MW:59kDa
Observed MW:70kDa

This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17.

Purification Method:Affinity purification
Gene ID:6198
Clone Number:ARC55792
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of NIH/3T3, using Phospho-p70 S6 kinase-T389 antibody (CABP1389) at 1:8000 dilution.NIH/3T3 cells were treated by 20% FBS at 37℃ for 20 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.

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