The Phospho-p70 S6 Kinase T389 Monoclonal Antibody is a powerful tool for studying the phosphorylation status of p70 S6 Kinase, a key signaling molecule involved in cell growth and proliferation. This antibody, monoclonal in nature, is specifically designed to target the phosphorylated threonine 389 residue of p70 S6 Kinase, allowing for precise detection and analysis in various experimental settings.Phosphorylation of p70 S6 Kinase at threonine 389 is a crucial event in the regulation of protein synthesis and cell growth, making this antibody an essential tool for researchers studying signal transduction pathways, cancer biology, and metabolic disorders.
Its high specificity and sensitivity in Western blot applications make it ideal for investigating the activation status of p70 S6 Kinase in cell lysates and tissue samples.By using the Phospho-p70 S6 Kinase T389 Monoclonal Antibody, researchers can gain valuable insights into the role of p70 S6 Kinase in various biological processes and diseases, paving the way for the development of targeted therapies and treatments. This antibody is an indispensable asset for studies in molecular biology, cell signaling, and drug discovery, providing accurate and reliable results for advanced research endeavors.
Product Name:
Phospho-p70 S6 kinase-T389 Monoclonal Antibody
SKU:
CABP1389
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around T389 of human p70 S6 kinase(NP_003152.1).
This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17.
Purification Method:
Affinity purification
Gene ID:
6198
Clone Number:
ARC55792
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of NIH/3T3, using Phospho-p70 S6 kinase-T389 antibody (CABP1389) at 1:8000 dilution.NIH/3T3 cells were treated by 20% FBS at 37℃ for 20 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
