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Phospho-p53-T55 Rabbit Monoclonal Antibody (CABP0986)

SKU:
CABP0986
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Monoclonal Antibody
Research Area:
Cell Death
€419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-p53-T55 Rabbit Monoclonal Antibody
SKU:CABP0986
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:A synthetic phosphorylated peptide around T55 of human p53 (P04637).
Sequence:WFTE D
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:P53; BCC7; LFS1; BMFS5; TRP53; Phospho-p53-T55
Positive Sample:293T， 293T + CA
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Endoplasmic reticulum, Mitochondrion matrix, Nucleus, PML body
Calculated MW:44kDa
Observed MW:53kDa

This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).

Purification Method:Affinity purification
Gene ID:7157
Clone Number:ARC1530
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of 293T cells, using Phospho-p53-T55 Rabbit mAb (CABP0986) at 1:1000 dilution.293T cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour or treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

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