The Phospho-p53 (T55) Rabbit Monoclonal Antibody (CABP0986) is a powerful tool for researchers studying the tumor suppressor protein p53 and its phosphorylation at threonine 55. This antibody, produced in rabbits, exhibits high specificity and sensitivity for detecting phospho-p53 (T55) in human samples, making it an invaluable asset for Western blot applications.Phosphorylation of p53 at threonine 55 plays a key role in regulating p53 activity and stability in response to various cellular stresses, including DNA damage and oncogenic signals. By targeting this specific phosphorylation site, researchers can gain insights into the intricate regulatory mechanisms that control p53 function and its impact on cell cycle progression, DNA repair, and apoptosis.
The Phospho-p53 (T55) Rabbit Monoclonal Antibody (CABP0986) opens up new avenues for exploring the role of p53 phosphorylation in cancer biology, aging, and other diseases where p53 dysregulation is a hallmark feature. Its reliable performance and reproducibility make it an indispensable tool for advancing our understanding of p53 signaling pathways and developing targeted therapies for p53-related disorders.
Product Name:
Phospho-p53-T55 Rabbit Monoclonal Antibody
SKU:
CABP0986
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around T55 of human p53 (P04637).
Sequence:
WFTE D
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
P53; BCC7; LFS1; BMFS5; TRP53; Phospho-p53-T55
Positive Sample:
293T， 293T + CA
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Endoplasmic reticulum, Mitochondrion matrix, Nucleus, PML body
Calculated MW:
44kDa
Observed MW:
53kDa
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).
Purification Method:
Affinity purification
Gene ID:
7157
Clone Number:
ARC1530
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of 293T cells, using Phospho-p53-T55 Rabbit mAb (CABP0986) at 1:1000 dilution.293T cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour or treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
