The Phospho-p53 (S9) Rabbit Monoclonal Antibody (CABP0985) is specifically designed for research related to phosphorylated p53 protein at serine 9, a key regulator of cell cycle progression and apoptosis. This antibody, derived from rabbits, has high specificity and sensitivity for human samples, and has been validated for use in Western blot applications. By binding to the phosphorylated form of p53 at serine 9, this antibody enables the detection and analysis of p53 activation in various cell types, making it an essential tool for studies in cancer biology, DNA damage response, and cell signaling pathways.Phosphorylation of p53 at serine 9 is known to influence its stability, activity, and ability to induce cell cycle arrest or apoptosis in response to cellular stress.
The role of phosphorylated p53 in regulating cell fate decisions and DNA repair mechanisms highlights its importance in maintaining genomic integrity and preventing the accumulation of mutations that can lead to tumorigenesis. Investigating the regulation of p53 phosphorylation at serine 9 is therefore crucial for understanding the signaling networks that control cellular responses to stress and may provide insights into potential targets for cancer therapy development.
Product Name:
Phospho-p53-S9 Rabbit Monoclonal Antibody
SKU:
CABP0985
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse
Immunogen:
A synthetic phosphorylated peptide around S9 of human p53 (P04637).
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).
Purification Method:
Affinity purification
Gene ID:
7157
Clone Number:
ARC1529
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of NIH/3T3 cells, using Phospho-p53-S9 Rabbit mAb (CABP0985) at 1:1000 dilution.NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 3min.
