The Phospho-p53 (S9) Polyclonal Antibody (CABP0085) is a powerful tool for researchers studying p53, a critical protein involved in cellular response to stress and DNA damage. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards human samples, making it an ideal choice for Western blot analysis.Phosphorylation of p53 at Serine 9 is known to play a crucial role in its activation and downstream signaling pathways. By targeting this specific phosphorylation site, researchers can gain valuable insights into the mechanisms governing p53 activation and its impact on cell cycle regulation, apoptosis, and DNA repair processes.The Phospho-p53 (S9) Polyclonal Antibody is a valuable asset for studies in cancer biology, as dysregulation of the p53 pathway is a common feature in many types of cancer.
By accurately detecting and quantifying phospho-p53 (S9) levels in various cell types, researchers can further our understanding of how p53 functions as a tumor suppressor and its potential as a target for cancer therapy.Overall, this antibody provides a reliable tool for investigating the role of phospho-p53 (S9) in cellular stress responses, DNA damage repair, and cancer development, making it an essential component of any research project in the fields of oncology and cell biology.
Product Name:
Phospho-p53-S9 Rabbit Polyclonal Antibody
SKU:
CABP0085
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around S9 of human p53 (NP_000537.3).
Sequence:
DPSV E
Tested Applications:
WBIPELISA
Recommended Dilution:
WB,1:500 - 1:2000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).
Purification Method:
Affinity purification
Gene ID:
7157
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from 293 and HT-29 cells, using Phospho-p53-S9 Rabbit pAb (CABP0085) at 1:1000 dilution. 293 cells were treated by UV for 15-30 minutes. HT-29 cells were treated by UV for 15-30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.
